MYSM1 rabbit pAb

• Background:

  Catalytic activity:Ubiquitin C-terminal thioester + H (2) O = ubiquitin + a thiol., domain:Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA., function:Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR) -induced gene activation., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the peptidase M67A family. MYSM1 subfamily., similarity:Contains 1 MPN (JAB/Mov34) domain., similarity:Contains 1 SANT domain., similarity:Contains 1 SWIRM domain., subunit:Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones.

• Description:

  Catalytic activity: Ubiquitin C-terminal thioester + H (2)O = ubiquitin + a thiol. Domain: Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA. function: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Belongs to the peptidase M67A family. MYSM1 subfamily. similarity: Contains 1 MPN (JAB/Mov34) domain. similarity: Contains 1 SANT domain. similarity: Contains 1 SWIRM domain. subunit: Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones.

• UniProt:

  Q5VVJ2

• Swiss Prot:

  Q5VVJ2

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human MYSM1. AA range:520-569

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  95kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  95kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus . Cytoplasm . Localizes to the cytoplasm in response to bacterial infection. .

• Other Product Names:

  MYSM1; KIAA1915; Histone H2A deubiquitinase MYSM1; 2A-DUB; Myb-like; SWIRM and MPN domain-containing protein 1

• Gene ID (Human):

  114803