Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit Monoclonal Antibody [JE59-46]

Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit Monoclonal Antibody [JE59-46]

• Description: Smads (or SMADs) comprise a family of structurally similar proteins that are the main signal transducers for receptors of the transforming growth factor beta (TGF-B) superfamily, which are critically important for regulating cell development and growth. There are three distinct sub-types of Smads: receptor-regulated Smads (R-Smads), common partner Smads (Co-Smads), and inhibitory Smads (I-Smads). The eight members of the Smad family are divided among these three groups. Trimers of two receptor-regulated SMADs and one co-SMAD act as transcription factors that regulate the expression of certain genes. The R-Smads consist of Smad1, Smad2, Smad3, Smad5 and Smad8/9, and are involved in direct signaling from the TGF-B receptor. R/Co-Smads are primarily located in the cytoplasm, but accumulate in the nucleus following TGF-β signaling, where they can bind to DNA and regulate transcription. However, I-Smads are predominantly found in the nucleus, where they can act as direct transcriptional regulators.
• Product Name Alternative: BSP-1 antibody BSP1 antibody HsMAD1 antibody JV4-1 antibody JV41 antibody MAD homolog 1 antibody MAD mothers against decapentaplegic homolog 1 antibody Mad related protein 1 antibody Mad-related protein 1 antibody MADH1 antibody MADR1 antibody Mothers against decapentaplegic homolog 1 antibody Mothers against DPP homolog 1 antibody SMA- AND MAD-RELATED PROTEIN 1 antibody SMAD 1 antibody SMAD family member 1 antibody SMAD mothers against DPP homolog 1 antibody Smad1 antibody SMAD1_HUMAN antibody TGF beta signaling protein 1 antibody Transforming growth factor-beta-signaling protein 1 antibody DKFZp781C1895 antibody DKFZp781O1323 antibody Dwfc antibody hSmad5 antibody JV5 1 antibody JV5-1 antibody MAD homolog 5 antibody MAD, mothers against decapentaplegic homolog 5 antibody MADH 5 antibody MADH5 antibody Mothers against decapentaplegic homolog 5 antibody mothers against decapentaplegic, drosophila, homolog of, 5 antibody Mothers against DPP homolog 5 antibody MusMLP antibody SMA and MAD related protein 5 antibody SMAD 5 antibody SMAD family member 5 antibody SMAD, mothers against DPP homolog 5 antibody Smad5 antibody SMAD5_HUMAN antibody MAD homolog 9 antibody Madh6 antibody Mothers against decapentaplegic antibody Mothers against decapentaplegic homolog 9 antibody Mothers against DPP homolog 9 antibody SMAD 9 antibody SMAD family member 9 antibody SMAD, mothers against DPP homolog 9 (Drosophila) antibody SMAD8A antibody SMAD8B antibody Smad9 antibody SMAD9_HUMAN antibody
• Abbreviation: SMAD1, BSP1, MADH1, MADR1, SMAD5, MADH5, SMAD9, MADH6, MADH9, SMAD8
• CAS Number: 9000-83-3
• Swiss Prot: Q15797 Human, Q99717 Human, O15198 Human, P70340 Mouse, P97454 Mouse, Q9JIW5 Mouse, O54835 Rat, P97588 Rat, Q9R1V3 Rat
• Cellular Locus: Cytoplasm, Nucleus, Mitochondrion.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD1 and SMAD5 protein.
• Isotype: IgG
• Clone: JE59-46
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC, IF-Cell
• Positive Control: HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate, NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate, human testis tissue, C6 treated with 10ng/mL BMP4 for 1 hour cell lysate, HeLa cells treated with 10ng/mL BMP4 for 1 hour, NIH/3T3 cells treated with 10ng/mL BMP4 for 1 hour.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC: 1:1,000 ;IF-Cell: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 52 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes