Phospho-SMAD2 (S465+467) Recombinant Rabbit Monoclonal Antibody [PSH05-76]

Phospho-SMAD2 (S465+467) Recombinant Rabbit Monoclonal Antibody [PSH05-76]

• Description: R-SMADs are receptor-regulated SMADs. SMADs are transcription factors that transduce extracellular TGF-β superfamily ligand signaling from cell membrane bound TGF-β receptors into the nucleus where they activate transcription TGF-β target genes. R-SMADS are directly phosphorylated on their c-terminus by type 1 TGF-β receptors through their intracellular kinase domain, leading to R-SMAD activation. R-SMADS include SMAD2 and SMAD3 from the TGF-β/Activin/Nodal branch, and SMAD1, SMAD5 and SMAD8 from the BMP/GDP branch of TGF-β signaling. In response to signals by the TGF-β superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.
• Product Name Alternative: Drosophila, homolog of, MADR2 antibody hMAD-2 antibody HsMAD2 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mother against DPP homolog 2 antibody Mothers against decapentaplegic homolog 2 antibody Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody Mothers against DPP homolog 2 antibody OTTHUMP00000163489 antibody Sma and Mad related protein 2 antibody Sma- and Mad-related protein 2 MAD antibody SMAD 2 antibody SMAD family member 2 antibody SMAD, mothers against DPP homolog 2 antibody SMAD2 antibody SMAD2_HUMAN antibody
• Abbreviation: SMAD2, MADH2, MADR2
• CAS Number: 9000-83-3
• Swiss Prot: Q15796 Human, Q62432 Mouse, O70436 Rat
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser465 and 467 of Human SMAD2.
• Isotype: IgG
• Clone: PSH05-76
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: HeLa cell lysate, HeLa starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, HeLa cells treated with 20ng/mL TGF-β1 for 15 minutes, HeLa, NIH/3T3, human stomach tissue, rat lung tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:500 ;IHC-P: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 52/48 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes