Recombinant Sortase A Protease

• Description:

  Recombinant Sortase A Protease_x000D_ Catalog number: B2011493_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 10 µg_x000D_ Molecular Weight or Concentration: 50 µg/mL_x000D_ Supplied as: Solution_x000D_ Applications: molecular tool for various biochemical applications_x000D_ Storage: -80 °C_x000D_ Keywords: Sortase A protease, recombinant_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Chen I, Dorr BM, Liu DR. A general strategy for the evolution of bond-forming enzymes using yeast display Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404._x000D_ 2: Stanborough T, Suryadinata R, Fegan N, Powell SM, Tamplin M, Nuttall SD, Chandry PS. Characterisation of the Brochothrix thermosphacta sortase A enzyme FEMS Microbiol Lett. 2018 Sep 1;365(17)._x000D_ 3: Sarpong K, Bose R. Efficient sortase-mediated N-terminal labeling of TEV protease cleaved recombinant proteins Anal Biochem. 2017 Mar 15;521:55-58._x000D_ 4: Westerlund K, Myrhammar A, Tano H, Gestin M, Karlström AE. Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization Molecules. 2021 May 12;26(10):2874._x000D_ 5: Bolscher JG, Oudhoff MJ, Nazmi K, Antos JM, Guimaraes CP, Spooner E, Haney EF, Garcia Vallejo JJ, Vogel HJ, van't Hof W, Ploegh HL, Veerman EC. Sortase A as a tool for high-yield histatin cyclization FASEB J. 2011 Aug;25(8):2650-8._x000D_ 6: Stanger K, Maurer T, Kaluarachchi H, Coons M, Franke Y, Hannoush RN. Backbone cyclization of a recombinant cystine-knot peptide by engineered Sortase A FEBS Lett. 2014 Nov 28;588(23):4487-96._x000D_ 7: Wójcik M, Szala K, van Merkerk R, Quax WJ, Boersma YL. Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting Proteins. 2020 Nov;88(11):1394-1400._x000D_ 8: Xu Z, Rivera-Hernandez T, Chatterjee O, Walker MJ, Moyle PM. Semisynthetic, self-adjuvanting vaccine development: Efficient, site-specific sortase A-mediated conjugation of Toll-like receptor 2 ligand FSL-1 to recombinant protein antigens under native conditions and application to a model group A streptococcal vaccine J Control Release. 2020 Jan 10;317:96-108._x000D_ 9: Jin M, Chen Y, Zhao Y, Che L, Ma Y, Li J, Wang Y, Tao H, Ma J, Pan B, Liu C, Huang P. Sortase A-aided Escherichia coli expression system for functional osteoprotegerin cysteine-rich domain Appl Microbiol Biotechnol. 2017 Jun;101(12):4923-4933._x000D_ 10: Li H, Chen Y, Zhang B, Niu X, Song M, Luo Z, Lu G, Liu B, Zhao X, Wang J, Deng X. Inhibition of sortase A by chalcone prevents Listeria monocytogenes infection Biochem Pharmacol. 2016 Apr 15;106:19-29. _x000D_ _x000D_ Products Related to Recombinant Sortase A Protease can be found at Proteins

• Short Description:

  Catalog Number: B2011493 (10 µg)_x000D_ Recombinant Sortase A Protease is a high quality sortase A from Staphylcoccus aureus expressed in E. coli. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height :

  0.5

• CAS Number:

  9000-83-3