Mouse anti MUC1 (CD227)

• Background:

  Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.

• UniProt:

  P15941

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  175C5

• Type:

  Primary Antibodies

• Applications:

  Electron microscopy, ELISA, Flow Cytometry, IF, IHC (frozen), IP, WB

• Field of Research:

  Cancer, Cell adhesion, CD Markers

• Assay Principle:

  140 H2 is useful for ELISA, immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration.

• Form:

  Each vial contains 100 µL 1 mg/mL purified monoclonal antibody in PBS containing 0,09% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• References & Citations:

  1. J. Hilkens, J. Wesseling, H. L. Vos, J. STorm, M. Boer, S. W. van der Valk and M. C. E. Maas, Episialin/CA15-3: its structure and involvement in breast cancer progression, Ned Tijdschr Klin Chem 1995; 20: 293-298

• Storage Conditions:

  The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product

• CAS Number:

  9007-83-4