Recombinant Mouse anti Human CD56

• Background:

  NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.

• Synonyms:

  CD56, Neural Cell Adhesion Molecule, NCAM

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG2b

• Clone:

  C5.9 E1 (derived from clone C5.9)

• Type:

  Primary Antibodies

• Source:

  Mouse monoclonal antibody 0566P is a recombinant CD56 monoclonal antibody produced in mammalian HEK293 cells. _x000D_ It is produced by cloning the antibody sequence of the mouse anti CD56 hybridoma C5.9, which was derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with an extract from the KG1a cell line.

• Applications:

  Flow Cytometry, ICC, IHC (frozen), IHC (paraffin)

• Field of Research:

  CD markers, Flow Cytometry, Immunology, Neuroscience

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  0566P is suitable for flow cytometry, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration; recommended range is 1:200 – 1:500 for immunohistochemistry with fluorochrome conjµgated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

• Stability:

  See expiration date on vial

• Concentration:

  1 mg/mL

• Form:

  Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0,09% sodium azide_x000D_ The purity of the antibody is >95% as determined by SDS-Polyacrylamide gel electrophoresis._x000D_ The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.

• References & Citations:

  1. Hercend T., Griffin J.D., Benussan A., Schmidt R.E., Edson M.A., Brennen A., Murray C., Daley J.F., Schlossman S.F., and Ritz J. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. J. Clin. Invest. 1985,75: 932. _x000D_ _x000D_ 2. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood NK Cells and Cytotoxic T Lymphocytes. J Immunol. 1986, 136: 4480. _x000D_ _x000D_ 3. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. J Exp Med 1986, 164: 351-356 _x000D_ _x000D_ 4. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Immunol 1987, 139: 3306-3319 _x000D_ _x000D_ 5. Hercend T. and Schmidt R.E. Characteristics and uses of natural killer cells. Immunol Today 1988, 9: 291-293. _x000D_ _x000D_ 6. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, J Clinical Endocrinology , Metabolism. 2003, 88: 3921-3930,

• Shipping Conditions:

  Ambient Temperature; freeze upon arrival

• Storage Conditions:

  The product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• CAS Number:

  9000-83-3