Mouse anti Human CD56

• Background:

  NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.

• Synonyms:

  CD56, NCAM, Neural Cell Adhesion Molecule

• UniProt:

  P13591

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG2b

• Clone:

  C5.9

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with an extract of the KG1a cell line.

• Applications:

  IHC (paraffin), Flow Cytometry, ICC

• Field of Research:

  Flow Cytometry

• Purification Method:

  ProteinG Chromatography

• Assay Principle:

  Antibody can be used for Immunohistochemistry (2-5 µg/mL, formalin-fixed, paraffin embedded tissues). Optimal concentration should be evaluated by serial dilutions.

• Stability:

  Reagents are stable for the period shown on the vial label when stored properly

• Concentration:

  See vial for Concentration

• Form:

  Purified monoclonal antibody clone C5.9, filtered, in PBS containing 0,08% azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Hercend T., Griffin J.D., Benussan A., Schmidt R.E., Edson M.A., Brennen A., Murray C., Daley J.F., Schlossman S.F., and Ritz J. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. J. Clin. Invest. 1985,75: 932. _x000D_ _x000D_ 2. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood NK Cells and Cytotoxic T Lymphocytes. J Immunol. 1986, 136: 4480. _x000D_ _x000D_ 3. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. J Exp Med 1986, 164: 351-356 _x000D_ _x000D_ 4. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Immunol 1987, 139: 3306-3319 _x000D_ _x000D_ 5. Hercend T. and Schmidt R.E. Characteristics and uses of natural killer cells. Immunol Today 1988, 9: 291-293. _x000D_ _x000D_ 6. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, J Clinical Endocrinology , Metabolism. 2003, 88: 3921-3930,_x000D_ _x000D_ PRODUCT SPECIFIC REFERENCES_x000D_ 1. Horikawa, M., et al. Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity. Journal of Investigative Dermatology 2005, 125: 731-737_x000D_ _x000D_ 2. Ishimoto, H., et al. Midkine, a heparin-binding growth factor, selectively stimulates proliferation of definitive zone cells of the Human fetal adrenal gland. Journal of Endocrinol. Metab 2006, 91: 4050-4056_x000D_ _x000D_ 3. Muench, M., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland. Journal of Clinical Endocrinol. Metab 2003, 88: 3921-3930_x000D_ _x000D_ 4. Miles, L.A., et al. Cell-Surface Actin Binds Plasminogen and Modulates Neurotransmitter Release from Catecholaminergic Cells. Journal of Neuroscience 2006, 26: 13017-13024

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  Flow Cytometry

• CAS Number:

  9007-83-4