Mouse anti Human Calpain I (large subunit, p80)

Mouse anti Human Calpain I (large subunit, p80)

• Background: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kD subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (uM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
• Synonyms: Mouse Anti-Calpain I, u-Calpain (Large subunit, p80), Monoclonal Antibody
• CAS Number: 9007-83-4
• UniProt: P07384
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat, Bovine
• Isotype: IgG1
• Clone: 15C10
• Conjugation: Unconjugated
• Type: Monoclonal Antibody
• Source: Hybridoma produced by the fusion of splenocytes from mice immunized with Human calpain I purified from placenta with mouse myeloma.
• Applications: WB, IP, Enzyme Immunoassay
• Field of Research: Cellular Structures
• Purification Method: Protein A/G Chromatography
• Assay Principle: Use at 0.5-1 µg/mL for ELISA and Western blot, 1-2µg/mL for immunoprecipitation.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as a solution containing phosphate buffered saline, 0,08% sodium azide and 50% glycerol.
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-48_x000D_ 2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB