AF488 NHS ester

• Description :

  AF488 NHS ester is an amine specific fluorescence probe (Em=525 nm) . AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization[1].

• CAS Number :

  [1374019-99-4]

• UNSPSC :

  12171500

• Hazard Statement :

  H302, H315, H319

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/af488-nhs-ester.html

• Purity :

  95.50

• Solubility :

  10 mM in DMSO

• Smiles :

  O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(ON5C(CCC5=O)=O)=O)=C1)O

• Molecular Formula :

  C25H17N3O13S2

• Molecular Weight :

  631.54

• Precautions :

  H302, H315, H319

• References & Citations :

  [1]Kendall CG, et al. Amine Analysis Using AlexaFluor 488 Succinimidyl Ester and Capillary Electrophoresis with Laser-Induced Fluorescence. J Anal Methods Chem. 2015;2015:368362.|[2]Lavoie RA, et al. Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery. Int J Mol Sci. 2019 Apr 8;20 (7) :1729.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  4°C (Powder, sealed storage, away from moisture and light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported