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Human Vitronectin, VTN ELISA Kit

CAT: 1360-BPE081-01Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1360-BPE081-01Size:96 Tests
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Background
Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
Product Name Alternative
Vitronectin
Synonyms
V75; VN
Gene Name
VTN
Gene ID
7448
UniProt
P04004
Target
Vitronectin
Field of Research
Cardiovascular, Immunology
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 1800 pg/ml | 2.896 | 2.863 | 2.745 2.831 900 pg/ml | 1.686 | 1.613 | 1.495 1.54 450 pg/ml | 0.947 | 0.893 | 0.775 0.839 225 pg/ml | 0.502 | 0.502 | 0.384 0.503 112.5 pg/ml | 0.323 | 0.321 | 0.203 0.32 56.25 pg/ml | 0.215 | 0.217 | 0.099 0.219 28.125 pg/ml | 0.16 | 0.168 | 0.05 0.177 0 pg/ml | 0.113 | 0.118 | 0 0.124
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
28.13-1800 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 320 | 17.5 | 5.5 Intra-assay | 2 | 20 | 640 | 16.6 | 2.6 Intra-assay | 3 | 20 | 1361 | 32.5 | 2.4 Inter-assay | 1 | 20 | 322 | 32.6 | 10.1 Inter-assay | 2 | 20 | 635 | 62.8 | 9.9 Inter-assay | 3 | 20 | 1357 | 123 | 9.1
Sensitivity
6.29 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
54306

UniProtKB · P04004

Vitronectin

VTNC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04004
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VTN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
VN
EC number
—
Processing
Precursor
Secondary accessions
B2R7G0, P01141, Q9BSH7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesion

PTM

Disulfide bondGlycoproteinPhosphoproteinSulfation

Molecular function

Heparin-binding

Domain

RepeatSignal

Cellular component

Secreted

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