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Human VTN (Vitronectin) ELISA Kit

CAT: 0855-ELK1744-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1744-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
UniProt
P04004
Reactivity
Human
Field of Research
Signal transduction; Metabolic pathway; Tumor immunity; Hepatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
400 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
6.25-400 ng/mL
Sensitivity
2.78 ng/mL

UniProtKB · P04004

Vitronectin

VTNC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04004
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VTN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
VN
EC number
—
Processing
Precursor
Secondary accessions
B2R7G0, P01141, Q9BSH7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesion

PTM

Disulfide bondGlycoproteinPhosphoproteinSulfation

Molecular function

Heparin-binding

Domain

RepeatSignal

Cellular component

Secreted

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