Acetylated-Lysine Recombinant Rabbit Monoclonal Antibody [PSH09-15]

Acetylated-Lysine Recombinant Rabbit Monoclonal Antibody [PSH09-15]

• Description: Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) . Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport.
• CAS Number: 9000-83-3
• Host: Rabbit
• Species Reactivity: Universal
• Immunogen: Synthetic Acetylated lysine-containing peptide.
• Isotype: IgG
• Clone: PSH09-15
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Tissue, ChIP
• Positive Control: HeLa cell lysate, HeLa treated with 1μM TSA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse liver tissue, rat liver tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IHC-P: 1:20,000-1:100,000 ;IF-Tissue:1:5,000-1:20,000 ;ChIP: Use 5 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes