Pan-Lactyl-lysine Recombinant Rabbit Monoclonal Antibody [PSH03-73]

Pan-Lactyl-lysine Recombinant Rabbit Monoclonal Antibody [PSH03-73]

• Description: Post-translational modifications (PTMs) represent a crucial means of regulating diverse biological processes and cellular physiology by influencing protein structure and function. Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300. This modification was high in gene promoter regions that lack acetylation and associated with activation of genes expression. As a large number of studies have demonstrated that lysine acylation possessed a diverse range of substrate proteins, however, no systematic analysis has been reported for lysine lactylation.
• Product Name Alternative: Lactyllysine antibody
• CAS Number: 9000-83-3
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Universal
• Immunogen: Synthetic Lactyl lysine-containing peptide.
• Isotype: IgG
• Clone: PSH03-73
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, FC, Dot Blot
• Positive Control: HeLa treated with 100mM Lactate sodium for 24 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue, HeLa cells treated with 100mM Lactate sodium for 24 hours.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IHC-P: 1:500-1:1,000 ;IF-Cell: 1:1,000 ;FC: 1:1,000 ;Dot Blot: 1:2,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes