QuicKey Pro Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit

QuicKey Pro Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit

• Abbreviation: EGFR
• Synonyms: ErbB-1; ErbB1; ERBB
• CAS Number: 7732-18-5
• UniProt: Q01279
• Cross Reactivity: This kit recognizes EGFR in samples.No significant cross-reactivity or interference between EGFR and analogues was observed.
• Target: Mouse
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cell Biology
• Detection Method: Colorimetric method; ELISA; Sandwich
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGFR. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse EGFR are added to the micro ELISA plate wells. Mouse EGFR in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse EGFR.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EGFR. The concentration of Mouse EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Mouse EGFR concentrations in serum, plasma and other biological fluids.