QuicKey Pro Mouse T (Testosterone) ELISA Kit

QuicKey Pro Mouse T (Testosterone) ELISA Kit

• Abbreviation: T
• Synonyms: Testosterone;T;
• CAS Number: 7732-18-5
• Species Reactivity: Mouse
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: Signal Transduction;Signal Transduction;Epigenetics and Nuclear Signaling;Developmental Biology
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Detection Range: 0.31-20 ng/mL
• Sensitivity: 0.14 ng/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months