EGFR Mouse mAb

EGFR Mouse mAb

• Description: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
• Synonyms: Epidermal Growth Factor Receptor ;ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog, Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
• Gene ID: 1956
• UniProt: P00533
• Cellular Locus: Secreted and Cell membrane. Endoplasmic reticulum membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Synthetic Peptide
• Clonality: Monoclonal
• Validated Applications: WB, IHC, IF
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1,000 IF 1;100-200  IHC 1:200-500
• Molecular Weight: 170kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.