Mouse anti Human CD71, conjugated with FITC

Mouse anti Human CD71, conjugated with FITC

• Background: CD71 is specific for the Human transferrin receptor, MW 190 kdaltons (kDa), 90 kDa reduced. CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.
• Synonyms: CD71 FITC
• CAS Number: 9007-83-4
• UniProt: P02786
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG1
• Clone: BGX.24
• Conjugation: FITC
• Type: Single-Color Reagent
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC. Antibodies should be stored at 4-8oC. Monoclonal antibodies should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.
• Stability: See expiration date on vial
• Concentration: Titered for flow cytometry
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Newman R, Schneider C, Sutherland R, Vodinelich L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982;1:397. 2. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press;1989:455-460. 3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1984;312:162-163. 4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980;152:1430. 5. Phillips JH, Le AM. Lanier LL. Natural killer cells activated in a Human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J Exp Med. 1984;159:993-1008. 6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of Human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.
• Shipping Conditions: Room Temperature
• Storage Conditions: Product should be stored at 4-8ºC; DO NOT FREEZE
• Functional Analysis: Flow Cytometry Staining