DNase I, RNase-free

CAT: 1465-EG22204SSize: 1000 UDry Ice: NoHazardous: No

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CAT#:1465-EG22204SSize:1000 U

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. The activity of DNase I is dependent on Ca 2+ , and can be activated by bivalent metal ions Mg 2+ , Mn 2+ , etc. In the presence of Mg 2+ , the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn 2+ , nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG22204-1.pdf

Concentration

1 U/μL

Components

• DNase I, RNase-free (1 U/μl) : 1000 U • 10xDNase I Buffer: 1.25 ml

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

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DNase I, RNase-free

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