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RNase A (DNase free)

CAT: 0013-GTR18878328Size: 2x 250 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18878328Size:2x 250 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.
Product Name Alternative
Endoribonuclease, Bovine pancreas
Concentration
Working concentration: 1-100 μg/ml (depending on application) . The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids.
Purity
≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate
Bioactivity
≥ 80 Kunitz units/mg
Form
Dry powder
Molecular Weight
Theoretical MW: 13.7 kDa (monomer)
Storage Conditions
Store at 2-8 °C
Notes
For research use only.
Applications Notes
Applications: 1) Plasmid and genomic DNA preparation 2) Removal of RNA from recombinant protein preparations 3) Ribonuclease protection assays 4) Mapping single-base mutations in DNA or RNA.

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