RNase A

CAT: 1465-EG20003SSize: 100 mgDry Ice: NoHazardous: No

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CAT#:1465-EG20003SSize:100 mg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. RNase A exhibits the highest activity in cleaving single-stranded RNA and demonstrates varying activities under different reaction conditions. At low salt concentrations (0~100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG20003.pdf

Components

• RNase A: 100 mg

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

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