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Recombinant e.coli GroES protein

CAT: 0112-GRS3002-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-GRS3002-020Size:20 µg
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Description
GroES protein is the co-chaperonin of GroES in E. coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.
Product Name Alternative
CPN10, GroES, 10 kDa chaperonin, groS, mopB, 10 kDa heat shock protein mitochondrial, Chaperonin 10, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, Heat shock 10kDa protein 1 chaperonin 10, HSP 10, HSP10, HSPE 1, HSPE1
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
10.4 kDa (97aa) confirmed by MALDI-TOF
Additionnal Information
GroES, CPN10, GroES, 10 kDa chaperonin, groS, mopB, 10 kDa heat shock protein mitochondrial, Chaperonin 10, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, Heat shock 10kDa protein 1 chaperonin 10, HSP 10, HSP10, HSPE 1, HSPE1, GRS3002-10 µg, GRS3002-20 µg, GRS3002-50 µg, GRS3002-100 µg, GRS3002-250 µg, GRS3002-500 µg, GRS3002-1 mg, GRS3002-10, GRS3002-20, GRS3002-50, GRS3002-100, GRS3002-250, GRS3002-500, GRS3002-1
References & Citations
Jue D. Wang , et al (1998) Proc. Natl. Acad. Sci. uSA 95, 12163-12168; ; Landry, S.J., et al (1993) Nature 364 , 255-258; ; Xu, Z., et al (1997) Nature 388,741-750;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5mM DTT, 10%glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_418566
Uniprot Accession Number
P0A6F9
Species
E.coli
AA Sequence
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA