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Recombinant human DUSP23 protein

CAT: 0112-ATGP3563-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3563-050Size:50 µg
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Description
DUSP23, also known as low molecular mass dual specificity phosphatase 3 (LDP-3), belongs to the protein-tyrosine phosphatase family. This protein is a protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. This protein able to enhance activation of JNK and p38 (MAPK14) . Recombinant human DUSP23 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 200 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Product Name Alternative
Dual specificity protein phosphatase 23, DuSP25, LDP-3, MOSP, VHZ, Low molecular mass dual specificity phosphatase 3, VH1-like phosphatase Z
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 200unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Molecular Weight
18.8 kDa (170aa) confirmed by MALDI-TOF
Additionnal Information
DUSP23, Dual specificity protein phosphatase 23, DUSP25, LDP-3, MOSP, VHZ, ATGP3563-10 µg, ATGP3563-20 µg, ATGP3563-50 µg, ATGP3563-100 µg, ATGP3563-250 µg, ATGP3563-500 µg, ATGP3563-1 mg, ATGP3563-10, ATGP3563-20, ATGP3563-50, ATGP3563-100, ATGP3563-250, ATGP3563-500, ATGP3563-1
References & Citations
Alonso A., et al. (2004) J. Biol. Chem. 279:35768-35774; ; WU Q, et al. (2004) Int J Biochem Cell Biol. 36 (8) :1542-53.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_060293
Uniprot Accession Number
Q9BVJ7
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGVQPPNFSW VLPGRLAGLA LPRLPAHYQF LLDLGVRHLV SLTERGPPHS DSCPGLTLHR LRIPDFCPPA PDQIDRFVQI VDEANARGEA VGVHCALGFG RTGTMLACYL VKERGLAAGD AIAEIRRLRP GSIETYEQEK AVFQFYQRTK

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