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Recombinant mouse GAPDH protein

CAT: 0112-ATGP3434-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3434-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Product Name Alternative
Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP, Peptidyl-cysteine S-nitrosylase GAPDH
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 40unit/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Molecular Weight
38.2 kDa (356aa) confirmed by MALDI-TOF
Additionnal Information
GAPDH, ATGP3434-10 µg, ATGP3434-20 µg, ATGP3434-50 µg, ATGP3434-100 µg, ATGP3434-250 µg, ATGP3434-500 µg, ATGP3434-1 mg, ATGP3434-10, ATGP3434-20, ATGP3434-50, ATGP3434-100, ATGP3434-250, ATGP3434-500, ATGP3434-1
References & Citations
Tarze A, Deniaud A, et al. (2007) . Oncogene. 26 (18) :2606-20.; ; Tisdale EJ, et al. (2007) . Traffic. 8 (6) :733-41.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_032110
Uniprot Accession Number
P16858
Species
Mouse
AA Sequence
MVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE

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