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Recombinant human GAPDH protein

CAT: 0112-GDH0801-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-GDH0801-500Size:500 µg
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Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
36 kDa (335aa) confirmed by MALDI-TOF
Additionnal Information
Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone
References & Citations
Ralser M., et al. (2007) .J Biol. 6 (4) :10; ; Tisdale Ej., et al. (2007) .Traffic. 8 (6) :733-41;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 1mM DTT, 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002037.2
Uniprot Accession Number
P04406
Species
Human
AA Sequence
MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

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