GAPDH antibody

• Description:

  Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

• Product Name Alternative:

  Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

• Host:

  Mouse

• Immunogen:

  GAPDH from rabbit muscle

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  AT4E8

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

• References & Citations:

  Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90.; ; Mazzola JL., et al, (2002) Neurotoxicology. 23 (4-5) :603-9.; ; Sirover MA. (1997) J Cell Biochem. 66 (2) :133-40.

• Other References:

  Chang Y, et al. Clinical impact of serum exosomal microRNA in liver fibrosis. (PLoS One. 2021) {https://pubmed.ncbi.nlm.nih.gov/34506494/}; ; Hah YS, et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. (Nutrients. 2022) {https://pubmed.ncbi.nlm.nih.gov/35889851/}; ; Hah YS, et al. Rutin Prevents Dexamethasone-Induced Muscle Loss in C2C12 Myotube and Mouse Model by Controlling FOXO3-Dependent Signaling. (Antioxidants. 2023) {https://pubmed.ncbi.nlm.nih.gov/36978887/}; ; Park SW, et al. Development of new tools to study membrane-anchored mammalian Atg8 proteins. (Autophagy. 2023) {https://pubmed.ncbi.nlm.nih.gov/36250672/}

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cancer

• NCBI Accession Number:

  NP_001075722.1

• Uniprot Accession Number:

  P46406

• WB Description:

  The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant-GAPDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A431 cell lysate; ; Lane 6.: LNCap cell lysate; ; Lane 7.: Ramos cell lysateThe tissue lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probedwith anti-GAPDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate; ; Lane 2.: Mouse Spleen tissue lysate; ; Lane 3.: Mouse Eye tissue lysate; ; Lane 4.: Mouse Muscle tissue lysate{ATGA0559-WB2.jpg}The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system.; ; Lane 1.: Anti-GAPDH monoclonal antibody (1:100,000) ; ; Lane 2.: Anti-GAPDH monoclonal antibody (1:50,000) ; ; Lane 3.: Anti-GAPDH monoclonal antibody (1:10,000) ; ; Lane 4.: Anti-GAPDH monoclonal antibody (1:1,000) ; ; {ATGA0559-WB3.jpg}

• IF Description:

  ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0559 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .