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Recombinant human PGAM2 protein

CAT: 0112-ATGP3428-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3428-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Specifications
Specific activity is >100units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
Product Name Alternative
Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >100unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
Molecular Weight
30.9 kDa (273aa) confirmed by MALDI-TOF
Additionnal Information
PGAM2, Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM, ATGP3428-10 µg, ATGP3428-20 µg, ATGP3428-50 µg, ATGP3428-100 µg, ATGP3428-250 µg, ATGP3428-500 µg, ATGP3428-1 mg, ATGP3428-10, ATGP3428-20, ATGP3428-50, ATGP3428-100, ATGP3428-250, ATGP3428-500, ATGP3428-1
References & Citations
Tsujino S., et al. (1989) J. Biol. Chem. 264:15334-15337; ; Hadjigeorgiou G.M., et al. (1999) Neuromuscul. Disord. 9:399-402
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_000281
Uniprot Accession Number
P15259
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK

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