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Human PGAM2 antibody

CAT: 0112-ATGA0399-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0399-100Size:100 µL
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Description
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Since both 3-PGA and 2-PGA are allosteric regulators of the pentose phosphate pathway (PPP) and glycine and serine synthesis pathways, respectively, PGAM2 may contribute to the biosynthesis of amino acids, 5-carbon sugar, and nucleotides precursors. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Mutations in this gene cause muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X. PGAM2 is one of two PGAM subunits found in humans and is predominantly expressed in adult muscle.
Product Name Alternative
Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PGAM2 (1-253aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT5A7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PGAM2, ATGA0399-10 µg, ATGA0399-20 µg, ATGA0399-50 µg, ATGA0399-100 µg, ATGA0399-250 µg, ATGA0399-500 µg, ATGA0399-1 mg, ATGA0399-10, ATGA0399-20, ATGA0399-50, ATGA0399-100, ATGA0399-250, ATGA0399-500, ATGA0399-1
References & Citations
Tsujino S., et al. (1986) The Journal of biological chemistry. 264 (26) : 15334-7.; ; Xu Y., et al. (2014) Cancer research. 74 (13) : 3630-42.; ; Tsujino S., et al. (1993) American journal of human genetics. 52 (3) : 472-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000281
Uniprot Accession Number
P15259
WB Description
The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombnant protein (50ng) ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : HepG2 cell lysate; ; Lane 4. : 293T cell lysate ; ; Lane 5. : Jurkat cell lysate; ; Lane 6. : NIH3T3 cell lysate; ; Lane 7. : A549 cell lysate; ; Lane 8. : MCF7 cell lysate
IF Description
ICC/IF analysis of PGAM2 in Hep3B cells. The cell was stained with ATGA0399 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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