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Recombinant human S100B protein

CAT: 0112-ATGP0628-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0628-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Recombinant human S100B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
S100 calcium binding protein B, S100 calcium binding protein beta neural, S100beta, S100 protein beta chain, S100 protein subunit beta, S100 calcium-binding protein B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.8 kDa (112aa) confirmed by MALDI-TOF
Additionnal Information
S-100beta, S100beta, S-100B, S100B, S-100 protein subunit beta, S100 protein subunit beta, S-100 protein beta subunit, S-100 protein beta chain, S100 protein beta chain, S-100 calcium-binding protein B, S100 calcium-binding protein B, S-100 calcium binding protein beta neural, S100 calcium binding protein beta neural, S-100 calcium binding protein beta chain, S100 calcium binding protein beta chain, S-100 calcium binding protein beta, S100 calcium binding protein beta, S-100 calcium binding protein B, S100 calcium binding protein B, S-100 beta, S100 beta, S-100, S100, S 100 protein beta subunit, S 100 protein beta chain, Protein S-100-B, Protein S100-B, Protein S-100 B, Protein S100 B, NEF, ATGP0628-10 µg, ATGP0628-20 µg, ATGP0628-50 µg, ATGP0628-100 µg, ATGP0628-250 µg, ATGP0628-500 µg, ATGP0628-1 mg, ATGP0628-010, ATGP0628-020, ATGP0628-050, ATGP0628-100, ATGP0628-250, ATGP0628-500, ATGP0628-01M
References & Citations
Van Dieck J., et al. (2010) Oncogene. 29 (14) :2024-35. ; ; Ayilavarapu S., et al. (2010) J Immunol. 184 (3) :1507-15.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_006263
Uniprot Accession Number
P04271
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE

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