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Recombinant E.coli trxB protein

CAT: 0112-ATGP0540-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0540-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.
Product Name Alternative
Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD (P) -binding, TRXR
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.
Molecular Weight
34.6 kDa (321aa) confirmed by MALDI-TOF
Additionnal Information
TRXB, Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR., ATGP0540-10 µg, ATGP0540-20 µg, ATGP0540-50 µg, ATGP0540-100 µg, ATGP0540-250 µg, ATGP0540-500 µg, ATGP0540-1 mg, ATGP0540-10, ATGP0540-20, ATGP0540-50, ATGP0540-100, ATGP0540-250, ATGP0540-500, ATGP0540-1
References & Citations
Faulkner MJ., et al. (2008) Proc Natl Acad Sci u S A. 105 (18) :6735-40. ; ; Takahata M., et al. (2008) J Biochem. 143 (4) :467-73.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
YP_003044110.1
Uniprot Accession Number
P0A9P4
Species
E.coli
AA Sequence
MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K

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