Recombinant E.coli dsbA protein

• Description:

  Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids) . Recombinant DsbA (residues 20-208) was expressed in E. coliand purified by using conventional chromatography techniques.

• Product Name Alternative:

  Thiol disulfide interchange protein dsbA, rpbB, ppfA, dsf, DsDNA-binding protein A, dsbA, Double-stranded DNA-binding protein, Disulfide oxidoreductase A periplasmic protein disulfide isomerase I, Disulfide oxidoreductase A, Disulfide oxidoreductase (DsbA) E.coli

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  Non-Tagged

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  21.2 kDa (190aa) confirmed by MALDI-TOF

• Additionnal Information:

  Disulfide oxidoreductase, DsbA, Disulfide oxidoreductase A, dsbA, rpbB, Disulfide oxidoreductase (DsbA) E Coli, DsDNA-binding protein A, Double-stranded DNA-binding protein, Disulfide oxidoreductase A periplasmic protein disulfide isomerase I, Thiol disulfide interchange protein dsbA., HSP0601-10 µg, HSP0601-20 µg, HSP0601-50 µg, HSP0601-100 µg, HSP0601-250 µg, HSP0601-500 µg, HSP0601-1 mg, HSP0601-10, HSP0601-20, HSP0601-50, HSP0601-100, HSP0601-250, HSP0601-500, HSP0601-1

• References & Citations:

  Charbonnier, J. B. et al. (1999) . Protein Sci. 8:96-105; ; Inaba, K. et al. (2004) . J. Biol. Chem. 279:6761-6768.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA

• Scientific Category:

  Heat Shock Proteins

• NCBI Accession Number:

  NP_418297

• Uniprot Accession Number:

  P0AEG4

• Species:

  Human

• AA Sequence:

  MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK