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L-929 Lysate

CAT: 0112-ATGL0031-200Size: 200 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGL0031-200Size:200 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
L-929 mouse fibroblast cells line established by W. R Earle in 1940. This strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+. L929 cells are used in studies of TNF-alpha, TNF-beta bioassays and cytokine assays.
Antigen Species
Mouse
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
L929, ATGL0031-10 µg, ATGL0031-20 µg, ATGL0031-50 µg, ATGL0031-100 µg, ATGL0031-250 µg, ATGL0031-500 µg, ATGL0031-1 mg, ATGL0031-10, ATGL0031-20, ATGL0031-50, ATGL0031-100, ATGL0031-250, ATGL0031-500, ATGL0031-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Mouse
WB Description
All lanes : L-929 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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