Jurkat Lysate

CAT: 0112-ATGL0003-200Size: 200 µgDry Ice: NoHazardous: No

CAT#:0112-ATGL0003-200Size:200 µg

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AVAILABILITY: InStock

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Jurkat cells are an immortalized line of human T lymphocyte cells take from the peripheral blood of a 14-year-old boy with T cell leukemia, in 1970s. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.

Antigen Species

Human

Applications

Concentration

2 mg/mL (determined by BCA assay)

Additionnal Information

Jurkat, ATGL0003-10 µg, ATGL0003-20 µg, ATGL0003-50 µg, ATGL0003-100 µg, ATGL0003-250 µg, ATGL0003-500 µg, ATGL0003-1 mg, ATGL0003-10, ATGL0003-20, ATGL0003-50, ATGL0003-100, ATGL0003-250, ATGL0003-500, ATGL0003-1

Storage Conditions

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Scientific Category

Lysates

Species

Human

WB Description

All lanes : Jurkat Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Preparation

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Lysate QC

Western Blot analyzed by both GAPDH and Beta tubulin antibody

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Jurkat Lysate

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