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Human PCBP1 antibody

CAT: 0112-ATGA0582-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0582-050Size:50 µL
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Description
PCBP-1 and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. PCBP1 constitutes a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA.
Product Name Alternative
Poly (rC) binding protein 1, Poly (rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PCBP1 (1-163aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT2A10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PCBP1, ATGA0582-10 µg, ATGA0582-20 µg, ATGA0582-50 µg, ATGA0582-100 µg, ATGA0582-250 µg, ATGA0582-500 µg, ATGA0582-1 mg, ATGA0582-10, ATGA0582-20, ATGA0582-50, ATGA0582-100, ATGA0582-250, ATGA0582-500, ATGA0582-1
References & Citations
Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24 (10) :1285-1292.; ; Leffers H., et al. (1995) Eur J Biochem 230 (2) :447-53.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006187
Uniprot Accession Number
Q15365
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: 293T cell lysate; ; Lane 5.: Jurkat cell lysate ; ; Lane 6.: MCF7 cell lysate
IF Description
ICC/IF analysis of PCBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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