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Human OAT antibody

CAT: 0112-ATGA0563-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0563-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.
Product Name Alternative
Ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human OAT (33-439aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT23A2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
OAT, ATGA0563-10 µg, ATGA0563-20 µg, ATGA0563-50 µg, ATGA0563-100 µg, ATGA0563-250 µg, ATGA0563-500 µg, ATGA0563-1 mg, ATGA0563-10, ATGA0563-20, ATGA0563-50, ATGA0563-100, ATGA0563-250, ATGA0563-500, ATGA0563-1
References & Citations
Stranska J, et al. (2008) Plant Signal Behav, 3 (11) :929-35.; ; Canas RA, et al. (2008) Plant Physiol, 148 (1) :77-88.; ; Levillain O, et al. (2007) Am J Physiol Renal Physiol, 292 (3) :F1016-27.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_000265
Uniprot Accession Number
P04181
WB Description
The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OAT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse liver tissue lysate; ; Lane 2.: 293T cell lysate
IF Description
ICC/IF analysis of OAT in HeLa cells. The cell was stained with ATGA0563 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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