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Recombinant human OAT protein

CAT: 0112-ATGP0458-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0458-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
OAT, also known as mitochondrial enzyme ornithine aminotransferase, is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. (L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.) Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Recombinant OAT protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase.
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
45.2 kDa (408aa) confirmed by MALDI-TOF
Additionnal Information
OAT, ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase., ATGP0458-10 µg, ATGP0458-20 µg, ATGP0458-50 µg, ATGP0458-100 µg, ATGP0458-250 µg, ATGP0458-500 µg, ATGP0458-1 mg, ATGP0458-10, ATGP0458-20, ATGP0458-50, ATGP0458-100, ATGP0458-250, ATGP0458-500, ATGP0458-1
References & Citations
Mitchell GA., et al. (1988) J. Biol. Chem. 263 (28) :14288-95.; ; Shah SA., et al. (1997) Structure. 5 (8) :1067-75;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000265.1
Uniprot Accession Number
P04181
Species
Human
AA Sequence
MTVQGPPTSD DIFEREYKYG AHNYHPLPVA LERGKGIYLW DVEGRKYFDF LSSYSAVNQG HCHPKIVNAL KSQVDKLTLT SRAFYNNVLG EYEEYITKLF NYHKVLPMNT GVEAGETACK LARKWGYTVK GIQKYKAKIV FAAGNFWGRT LSAISSSTDP TSYDGFGPFM PGFDIIPYND LPALERALQD PNVAAFMVEP IQGEAGVVVP DPGYLMGVRE LCTRHQVLFI ADEIQTGLAR TGRWLAVDYE NVRPDIVLLG KALSGGLYPV SAVLCDDDIM LTIKPGEHGS TYGGNPLGCR VAIAALEVLE EENLAENADK LGIILRNELM KLPSDVVTAV RGKGLLNAIV IKETKDWDAW KVCLRLRDNG LLAKPTHGDI IRFAPPLVIK EDELRESIEI INKTILSF

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