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Human VHL antibody

CAT: 0112-ATGA0542-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0542-050Size:50 µL
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Description
Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition todevelop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas andhemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in theVHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-typeallele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) anda smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL proteinis also involved in the degradation of hypoxia-inducible factor (HIF) .
Product Name Alternative
Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1, HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin bindingprotein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, vonHippel Lindau syndrome, von Hippel Lindau tumor suppressor, AVH0616
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human VHL (1-154aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT82B10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VHL, ATGA0542-10 µg, ATGA0542-20 µg, ATGA0542-50 µg, ATGA0542-100 µg, ATGA0542-250 µg, ATGA0542-500 µg, ATGA0542-1 mg, ATGA0542-10, ATGA0542-20, ATGA0542-50, ATGA0542-100, ATGA0542-250, ATGA0542-500, ATGA0542-1
References & Citations
Latif F., et al. (1993) Science. 260 (5112) : 1317-20.; ; Duan DR., et al. (1995) PNAS. 92 (14) : 6459-63.; ; Maxwell PH., et al. (1999) Nature. 399 (6733) : 271-5.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_000542
Uniprot Accession Number
P40337
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Raji cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: MCF7 cell lysate; ; Lane 7.: PC3 cell lysate;
IF Description
ICC/IF analysis of VHL in Hep3B cells. The cell was stained with ATGA0542 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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