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Human Vimentin antibody

CAT: 0112-ATGA0453-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0453-050Size:50 µL
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Description
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.
Product Name Alternative
Vimentin, epididymis luminal protein 113, HEL113, CTRCT30
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human VIM (1-466aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT5H8
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Vimentin, Vimentin, epididymis luminal protein 113, HEL113, CTRCT30, ATGA0453-10 µg, ATGA0453-20 µg, ATGA0453-50 µg, ATGA0453-100 µg, ATGA0453-250 µg, ATGA0453-500 µg, ATGA0453-1 mg, ATGA0453-10, ATGA0453-20, ATGA0453-50, ATGA0453-100, ATGA0453-250, ATGA0453-500, ATGA0453-1
References & Citations
John E. Eriksson., et al. (2009) J Clin Invest. 119 (7) : 1763-1771. ; ; Cabeen MT., et al. (2010) Annu Rev Genet. 44: 365-392.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_003371
Uniprot Accession Number
P08670
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Jurkat cell lysate ; ; Lane 2 : K562 cell lysate ; ; Lane 3 : 293T cell lysate ; ; Lane 4 : A549 cell lysate ; ; Lane 5 : SK-OV-3 cell lysate ; ; Lane 6 : HeLa cell lysate
IF Description
ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0453-IF1b.jpg}

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