Products for Research Use Only

Plant DNA Mega Kit

CAT: 0853-GD2615-00Size: 1 / BoxDry Ice: NoHazardous: No
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CAT#:0853-GD2615-00Size:1 / Box
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Plant DNA Mega Kit is engineered for ultra-high-capacity extraction of genomic DNA from large quantities of plant tissue—including leaves, roots, seeds or woody material—typically handling up to ~500 mg to 1 g of fresh tissue (or equivalent dried weight) . The protocol begins with mechanical disruption (liquid-nitrogen grinding or bead-beating), strong-lysis buffer treatment to break down plant cells and remove polysaccharides and polyphenols, followed by high-capacity DNA binding (up to ~500 μg or more) to a large-format column or bead matrix. Rigorous wash steps eliminate inhibitors common in plant materials such as phenolics and polysaccharides, and the purified DNA is eluted in low-salt buffer or water. Output DNA is ready for demanding downstream applications including long-range PCR, restriction digestion, sequencing, array-based genotyping and genome assembly workflows. The kit includes large-volume lysis/neutralization buffers, high-capacity binding columns or beads, wash buffers, optional RNase A, Elution Buffer, collection tubes and a detailed user protocol. No organic solvents or ethanol precipitation are required—making it ideal for plant-science, biotechnology and large-scale genomics labs.
Hazard Statement
N
Shipping Conditions
Ambient
Shelf Life
24
Latex Free Indicator
Latex Free