Human PPCS antibody

• Description:

  PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.

• Product Name Alternative:

  Phosphopantothenate--cysteine ligase, COAB, PPC synthetase

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human PPCS (1-311aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  AT10D11

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGA0443-10 µg, ATGA0443-20 µg, ATGA0443-50 µg, ATGA0443-100 µg, ATGA0443-250 µg, ATGA0443-500 µg, ATGA0443-1 mg, ATGA0443-10, ATGA0443-20, ATGA0443-50, ATGA0443-100, ATGA0443-250, ATGA0443-500, ATGA0443-1

• References & Citations:

  Manoj N., et al. (2003) Structure. 11: 927-936. ; ; Christina Spry., et al. (2009) J Biol Chem. 284: 24904-24913.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Signal Transduction

• NCBI Accession Number:

  NP_078940

• Uniprot Accession Number:

  Q9HAB8

• WB Description:

  The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPCS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: HeLa cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: SK-OV-3 cell lysate