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Recombinant human PPCS protein

CAT: 0112-ATGP1064-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1064-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Phosphopantothenate--cysteine ligase, COAB, PPC synthetase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
36.1 kDa (331aa) confirmed by MALDI-TOF
Additionnal Information
PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGP1064-10 µg, ATGP1064-20 µg, ATGP1064-50 µg, ATGP1064-100 µg, ATGP1064-250 µg, ATGP1064-500 µg, ATGP1064-1 mg, ATGP1064-10, ATGP1064-20, ATGP1064-50, ATGP1064-100, ATGP1064-250, ATGP1064-500, ATGP1064-1
References & Citations
Manoj N. et al. (2003) Structure. 11 : 927-936.; ; Christina Spry. et al. (2009) J Biol Chem. 284: 24904-24913.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_078940
Uniprot Accession Number
Q9HAB8
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N

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