Human PPCS antibody

CAT: 0112-ATGA0443-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0443-050Size:50 µL
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Description
PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
Product Name Alternative
Phosphopantothenate--cysteine ligase, COAB, PPC synthetase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PPCS (1-311aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT10D11
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGA0443-10 µg, ATGA0443-20 µg, ATGA0443-50 µg, ATGA0443-100 µg, ATGA0443-250 µg, ATGA0443-500 µg, ATGA0443-1 mg, ATGA0443-10, ATGA0443-20, ATGA0443-50, ATGA0443-100, ATGA0443-250, ATGA0443-500, ATGA0443-1
References & Citations
Manoj N., et al. (2003) Structure. 11: 927-936. ; ; Christina Spry., et al. (2009) J Biol Chem. 284: 24904-24913.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_078940
Uniprot Accession Number
Q9HAB8
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPCS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: HeLa cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: SK-OV-3 cell lysate

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