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Human SHMT1 antibody

CAT: 0112-ATGA0413-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0413-100Size:100 µL
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Description
Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.
Product Name Alternative
Serine hydroxymethyltransferase 1, CSHMT, SHMT
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SHMT1 (1-483aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT26E5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SHMT1, ATGA0413-10 µg, ATGA0413-20 µg, ATGA0413-50 µg, ATGA0413-100 µg, ATGA0413-250 µg, ATGA0413-500 µg, ATGA0413-1 mg, ATGA0413-10, ATGA0413-20, ATGA0413-50, ATGA0413-100, ATGA0413-250, ATGA0413-500, ATGA0413-1
References & Citations
Garrow TA., et al. (1993) J Biol Chem. 268 (16) : 11910-6.; ; Perry C., et al. (2005) J Biol Chem. 280 (1) : 396-400. ; ; Pinthong C., et al. (2014) FEBS J. 281 (11) : 2570-83.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
AAH07979
Uniprot Accession Number
P34896
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HeLa cell lysate; ; Lane 3.: NIH-3T3 cell lysate ; ; Lane 4.: LnCaP cell lysate
IF Description
ICC/IF analysis of SHMT1 in Hep3B cells. The cell was stained with ATGA0413 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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