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Human AK2 antibody

CAT: 0112-ATGA0393-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0393-050Size:50 µL
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Description
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants.
Product Name Alternative
Adenylate kinase 2, Mitochondrial Adenylate kinase 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, ADK2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human AK2 (1-239aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT7E7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Mitochondrial Adenylate kinase 2, EC2743, EC2.7.4.3, EC 2.7.4.3, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ATP AMP transphosphorylase 2, ATP AMP transphosphorylase, ATP AMP phosphotransferase, Ak-2, AK2, Ak 2, ADK2, ADK 2, Adenylate monophosphate kinase, Adenylate kinase2, Adenylate kinase isoenzyme 2, Adenylate kinase 2 mitochondrial isoform a, Adenylate kinase 2, ATGA0393-10 µg, ATGA0393-20 µg, ATGA0393-50 µg, ATGA0393-100 µg, ATGA0393-250 µg, ATGA0393-500 µg, ATGA0393-1 mg, ATGA0393-010, ATGA0393-020, ATGA0393-050, ATGA0393-100, ATGA0393-250, ATGA0393-500, ATGA0393-01M
References & Citations
Kim D Pruitt., et al. (2012) Nucleic Acids Res. 40 (Database issue) : D130-D135.; ; Lagresle-Peyrou C., et al. (2009) Nat Genet. 41 (1) : 106-11.; ; Lee Y., et al. (1998) J. Biochem. 123 (1) : 47-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Apoptosis
NCBI Accession Number
NP_001616
Uniprot Accession Number
P54819
WB Description
The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: NIH/3T3 cell lysate {ATGA0393-WB2.jpg}
IF Description
ICC/IF analysis of AK2 in HeLa cells. The cell was stained with ATGA0393 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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