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Recombinant mouse AK2 protein

CAT: 0112-ATGP3701-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3701-020Size:20 µg
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Description
Ak2, also known as Adenylate kinase 2 mitochondrial isoform a, is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Ak2 is localized in the mitochondrial intermembrane space and may pay a role in apoptosis. Recombinant mouse Ak2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 40 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Product Name Alternative
Adenylate kinase 2 mitochondrial isoform a, Ak-2, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 40unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Molecular Weight
29.0 kDa (263aa) confirmed by MALDI-TOF
Additionnal Information
Mitochondrial Adenylate kinase 2, EC2743, EC2.7.4.3, EC 2.7.4.3, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ATP AMP transphosphorylase 2, ATP AMP transphosphorylase, ATP AMP phosphotransferase, Ak-2, AK2, Ak 2, ADK2, ADK 2, Adenylate monophosphate kinase, Adenylate kinase2, Adenylate kinase isoenzyme 2, Adenylate kinase 2 mitochondrial isoform a, Adenylate kinase 2, ATGP3701-10 µg, ATGP3701-20 µg, ATGP3701-50 µg, ATGP3701-100 µg, ATGP3701-250 µg, ATGP3701-500 µg, ATGP3701-1 mg, ATGP3701-010, ATGP3701-020, ATGP3701-050, ATGP3701-100, ATGP3701-250, ATGP3701-500, ATGP3701-01M
References & Citations
Kohler C., et al. (1999) FEBS Lett. 447 (1) :10-2.; ; Lee Y. et al., (1998) J Biol chem. 123 (1) :47-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001029138
Uniprot Accession Number
Q9WTP6
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAPNVL ASEPEIPKGI RAVLLGPPGA GKGTQAPKLA ENFCVCHLAT GDMLRAMVAS GSELGKKLKA TMDAGKLVSD EMVVELIEKN LETPSCKNGF LLDGFPRTVR QAEMLDDLME KRKEKLDSVI EFSIQDSLLI RRITGRLIHP KSGRSYHEEF NPPKEPMKDD ITGEPLIRRS DDNEKALKTR LEAYHTQTTP LVEYYRKRGI HCAIDASQTP DIVFASILAA FSKATCKDLV MFI

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