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Human ACAT2 antibody

CAT: 0112-ATGA0335-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0335-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT (acetyl-Coenzyme A acetyltransferase 2) gene. ACAT2 is an acetyl-CoA C-acetyltransferase enzyme, plays a role in regulating lipid metabolism. It catalyzes the synthesis of acetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids.
Product Name Alternative
Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACAT2 (1-397aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT55D5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ACAT2, ATGA0335-10 µg, ATGA0335-20 µg, ATGA0335-50 µg, ATGA0335-100 µg, ATGA0335-250 µg, ATGA0335-500 µg, ATGA0335-1 mg, ATGA0335-10, ATGA0335-20, ATGA0335-50, ATGA0335-100, ATGA0335-250, ATGA0335-500, ATGA0335-1
References & Citations
Shintani S., et al. (1999) Genetics. 152 (2) : 743-754.; ; Middleton B., et al. (1974) Biochem J. 139 (1) : 109-121.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Actin & Myosin Related
NCBI Accession Number
NP_005882
Uniprot Accession Number
Q9BWD1
WB Description
The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human ACAT2 protein; ; The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: ACAT2 Transfected 293T cell lysate; ; {ATGA0335-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Ramos cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: HL-60 cell lysate; ; Lane 5.: SW480 cell lysate; ; {ATGA0335-WB3.jpg}
FACS Description
Flow cytometry analysis of ACAT2 in PC3 cells. The cell was stained with ATGA0335 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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