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Recombinant human ACAT2 protein

CAT: 0112-ATGP0634-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0634-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.4 kDa (433aa) confirmed by MALDI-TOF
Additionnal Information
ACAT2, Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic, ATGP0634-10 µg, ATGP0634-20 µg, ATGP0634-50 µg, ATGP0634-100 µg, ATGP0634-250 µg, ATGP0634-500 µg, ATGP0634-1 mg, ATGP0634-10, ATGP0634-20, ATGP0634-50, ATGP0634-100, ATGP0634-250, ATGP0634-500, ATGP0634-1
References & Citations
Alger HM., et al. (2010) J Biol Chem. 285 (19) :14267-74.; ; Bryleva EY., et al. (2010) Proc Natl Acad Sci u S A. 107 (7) :3081-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_005882
Uniprot Accession Number
Q9BWD1
Species
Human
AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE

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