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Human STOM antibody

CAT: 0112-ATGA0291-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0291-100Size:100 µL
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Description
STOM (stomatin) is a member of a highly conserved family of integral membrane proteins. This protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of STOM is associated with hereditary stomatocytosis, a form of hemolytic anemia. Although the wide distribution of stomatin and its constitutive expression suggest an important role for this protein in cell biology, perhaps as a house-keeping component, its function remains undetermined. The massive presence of stomatin in membrane-protruding folds and extensions suggests a possible structural role for this protein in the formation of these structures and/or the anchorage to the actin cytoskeleton.
Product Name Alternative
Erythrocyte band 7 integral membrane protein isoform a, BND7, EPB7, EPB72
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human STOM (55-288aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT33F5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
STOM, ATGA0291-10 µg, ATGA0291-20 µg, ATGA0291-50 µg, ATGA0291-100 µg, ATGA0291-250 µg, ATGA0291-500 µg, ATGA0291-1 mg, ATGA0291-10, ATGA0291-20, ATGA0291-50, ATGA0291-100, ATGA0291-250, ATGA0291-500, ATGA0291-1
References & Citations
Hiebl-Dirschmied CM., et al. (1991) Biochim Biophys Acta 1090 (1) : 123-4.; ; Stewart GW (1997) J. Biol. Chem. 29 (2) : 271-4.; ; Entrez Gene: STOM stomatin.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_004090
Uniprot Accession Number
P27105
WB Description
The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STOM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate; ; Lane 2.: STOM Transfected 293T cell lysate {ATGA0291-WB2.jpg}
IF Description
ICC/IF analysis of STOM in HeLa cells. The cell was stained with ATGA0291 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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