Human REXO2 antibody

CAT: 0112-ATGA0269-100Size: 100 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0269-100Size:100 µL
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Description
REX2, RNA exonuclease 2 homolog (S. cerevisiae), also known as REXO2, is an enzyme which in humans is encoded by the REXO2 gene. Nucleases are components of DNA and RNA metabolism that carry out functions in DNA repair that also may be involved in resistance of human cells to UV-C-induced cell death through its role in the DNA repair process, replication, and recombination and in RNA processing and degradation. SFN is a homolog of Orn, a 3-prime-to-5-prime exoribonuclease of E. coli that attacks the free 3-prime hydroxyl group on single-stranded RNA, releasing 5-prime mono-nucleotides in a sequential manner.
Product Name Alternative
Oligoribonuclease mitochondrial, Oligoribonuclease, mitochondrial, REX2, RNA exonuclease 2 homolog (S. cerevisiae), CGI-114, REX2, RFN, SFN
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human REXO 2 (26-237aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT59D9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
REXO2, Oligoribonuclease mitochondrial, Oligoribonuclease, mitochondrial, REX2, RNA exonuclease 2 homolog (S. cerevisiae), CGI-114, REX2, RFN, SFN, ATGA0269-10 µg, ATGA0269-20 µg, ATGA0269-50 µg, ATGA0269-100 µg, ATGA0269-250 µg, ATGA0269-500 µg, ATGA0269-1 mg, ATGA0269-10, ATGA0269-20, ATGA0269-50, ATGA0269-100, ATGA0269-250, ATGA0269-500, ATGA0269-1
References & Citations
Entrez Gene: REXO2 REX2, RNA exonuclease 2 homolog (S. cerevisiae) ; ; Lai, CH., et al. (2000) Genome Res 10 (5) : 703-13; ; Nguyen, LH., et al. (2000) J.Biol.Chem 275 (34) : 25900-6
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_056338
Uniprot Accession Number
Q9Y3B8
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human REXO2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : SW480 cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : Jurkat cell lyate ; ; Lane 5. : K562 cell lysate ; ; Lane 6. : NIH3T3 cell lysate
IF Description
ICC/IF analysis of REXO2 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human REXO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0269-IF1b.jpg} ICC/IF analysis of REXO2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human REXO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0269-IF2a.jpg} {ATGA0269-IF2b.jpg}

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