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Human PNMT antibody

CAT: 0112-ATGA0214-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0214-100Size:100 µL
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Description
Phenylethanolamine N-methyltransferase (PNMT) catalyzes the synthesis of epinephrine and plays an important role in regulating cardiovascular function. Expression of PNMT is regulated by hormonal and neural stimuli because its promoter contains sequences responding to cholinergic and depolarization stimuli. PNMT is the final enzyme in the catecholamine pathway, catalyzing the conversion of norepinephrine to epinephrine using as the cosubstrate and methyl donor, S-adenosylmethionine.
Product Name Alternative
Phenylethanolamine N-methyltransferase, PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PNMT (1-282aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT1C11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PNMT, ATGA0214-10 µg, ATGA0214-20 µg, ATGA0214-50 µg, ATGA0214-100 µg, ATGA0214-250 µg, ATGA0214-500 µg, ATGA0214-1 mg, ATGA0214-10, ATGA0214-20, ATGA0214-50, ATGA0214-100, ATGA0214-250, ATGA0214-500, ATGA0214-1
References & Citations
Wurtman R.J., et al. (1965) Science 150: 1464-1465.; ; Wurtman R.J., et al. (1966) J Biol Chem 241: 2301-2305.; ; Huang C., et al. (2011) lAmerican Journal of Hypertension 24: 1222-1226.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_002677
Uniprot Accession Number
P11086
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: PNMT transfected 293T cell lysate
IF Description
ICC/IF analysis of PNMT in Hep3B cells. The cell was stained with ATGA0214 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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