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Human PKLR antibody

CAT: 0112-ATGA0209-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0209-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
Product Name Alternative
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PKLR (47-574aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1E3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PKLR, ATGA0209-10 µg, ATGA0209-20 µg, ATGA0209-50 µg, ATGA0209-100 µg, ATGA0209-250 µg, ATGA0209-500 µg, ATGA0209-1 mg, ATGA0209-10, ATGA0209-20, ATGA0209-50, ATGA0209-100, ATGA0209-250, ATGA0209-500, ATGA0209-1
References & Citations
Meza NW., et al. (2009) . Mol Ther. 17 (12) :2000-9.; ; Roy MF., et al. (2007) . J Exp Med. 204 (12) :2949-61.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000289
Uniprot Accession Number
P30613
WB Description
The cell lysate of 293T (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Balb-3T3 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Raji cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A549 cell lysate {ATGA0209-WB2.jpg}
IF Description
ICC/IF analysis of PKLR in Hep3B cells. The cell was stained with ATGA0209 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PKLR in HeLa cells. The cell was stained with ATGA0209 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0209-IF2.jpg}

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